One of the central methodological insights of feminist science studies has been refuting the binary worlds of nature and culture. Over the last three decades, feminist science studies has developed a vibrant epistemological, and methodological apparatus for studying the natural world as a naturecultural world. Bringing together (inter)disciplinary methodologies, philosophies, practices, assumptions, methods and languages of women's studies and the natural sciences, we see the emergence of new modes of knowledge production. This paper explores the epistemological challenges of studying gender in the natural and physical sciences and the methodological tools the field has developed to study the human and non-human, life and non-life.